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Quantification of Hypopigmentation Activity In Vitro
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Penicillamine determination using a tyrosinase micro-rotating biosensor.

Angel A J Torriero1, Eloy Salinas, Eduardo J Marchevsky

  • 1Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera 5700, San Luis, Argentina. torriero@unsl.edu.ar

Analytica Chimica Acta
|August 29, 2007
PubMed
Summary
This summary is machine-generated.

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A novel biosensor uses immobilized tyrosinase to detect penicillamine (PA). The enzyme

Area of Science:

  • Biochemistry
  • Electrochemistry
  • Biosensor Technology

Background:

  • Tyrosinase enzyme catalyzes catechol oxidation to o-benzoquinone.
  • o-benzoquinone undergoes electrochemical reduction on glassy carbon electrodes.
  • Penicillamine (PA) reacts with o-benzoquinone via Michael addition.

Purpose of the Study:

  • To develop a sensitive amperometric biosensor for penicillamine determination.
  • To investigate the electrochemical reaction between o-benzoquinone and penicillamine.
  • To validate the biosensor for analyzing penicillamine in pharmaceutical and biological samples.

Main Methods:

  • Immobilization of tyrosinase on a rotating disk electrode.
  • Electrochemical detection of o-benzoquinone reduction current.

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  • Amperometric measurement of penicillamine concentration based on current decrease.
  • Linear regression analysis for calibration curve generation.

    • Main Results:

    • A linear relationship was observed between penicillamine concentration and the decrease in reduction current.
    • The linear range for penicillamine determination was 0.02–80 µM with a high correlation coefficient (r=0.999).
    • The developed biosensor demonstrated good reproducibility (R.S.D. < 4.0%) and stability (> 1 month).

    Conclusions:

    • The tyrosinase-based biosensor provides a sensitive and reliable method for penicillamine quantification.
    • The electrochemical detection mechanism involves the reaction of penicillamine with o-benzoquinone.
    • This biosensor is suitable for the determination of penicillamine in drug and synthetic serum samples.