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Updated: Jul 12, 2026

Preparation of High-Quality Fermented Fish Product
Published on: August 23, 2019
Seafood freshness determination through vapour phase Fourier transform infrared spectroscopy
S Armenta1, N M M Coelho, R Roda
1Department of Analytical Chemistry, Universitat de València, Edifici Jeroni Muñoz, 50th Dr. Moliner, 46100 Burjassot, València, Spain.
Abstract:
A new vapour-phase manifold has been developed to determine trimethylamine (TMA) in fish and cephalopod samples by Fourier transform infrared (FT-IR) spectroscopy. Samples were treated off-line for 1h with trichloroacetic acid (TCA), filtered and washed. The obtained extracts were aspirated and alkalinized with NaOH 2.0M, in an on-line system. TMA was separated from the solution in a gas phase separator and then transported by means of a nitrogen carrier into a home made 10 cm pathlength IR gas cell, where the corresponding FT-IR spectra were acquired by accumulating 30 scans per spectrum with 2 cm(-1) nominal resolution. The method was applied to the determination of TMA in natural samples providing concentration values statistically comparables with those obtained by a head space gas chromatography (HS-GC) reference procedure. The sample throughput by FT-IR is increased by a factor of 6 as compared with HS-GC.
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