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Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Published on: May 25, 2022

vanD and vanG-like gene clusters in a Ruminococcus species isolated from human bowel flora.

M-C Domingo¹, A Huletsky, R Giroux

¹Centre de Recherche en Infectiologie de l'Université Laval, CHUQ, 2705 boul. Laurier, Québec, Québec G1V 4G2, Canada.

Antimicrobial Agents and Chemotherapy

|August 29, 2007

Summary

A novel anaerobic bacterium, strain CCRI-16110, was identified harboring vancomycin resistance genes vanD and vanG. This discovery suggests anaerobic bacteria may act as reservoirs for these critical resistance genes in the human gut.

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A Murine Model of Group B Streptococcus Vaginal Colonization

A Murine Model of Group B Streptococcus Vaginal Colonization

Published on: November 16, 2016

Area of Science:

Microbiology
Genetics
Antimicrobial Resistance

Background:

Vancomycin-resistant enterococci (VRE) are a significant healthcare concern.
Hospital surveillance programs aim to detect carriers of VRE.
Anaerobic bacteria are increasingly recognized for their role in the human microbiome.

Purpose of the Study:

To identify and characterize a novel vancomycin-resistant anaerobic bacterium isolated from a human fecal specimen.
To investigate the genetic basis of vancomycin resistance in this novel isolate.
To assess the potential role of anaerobic bacteria as reservoirs for vancomycin resistance genes.

Main Methods:

Isolation and identification of anaerobic bacteria from human fecal samples.
16S rRNA gene sequencing for species identification.
Determination of vancomycin and teicoplanin resistance by MIC testing.
Whole-genome sequencing to analyze vancomycin resistance gene clusters (vanD and vanG-like).
Comparative sequence analysis of identified genes with known resistance determinants.

Main Results:

A vancomycin-resistant, anaerobic, gram-positive coccus (strain CCRI-16110) was isolated.
16S rRNA gene sequencing suggested a novel Ruminococcus species.
Strain CCRI-16110 exhibited high resistance to vancomycin and teicoplanin (MICs >256 µg/ml).
The vanD gene cluster, including vanD1 and intD, was identified and highly similar to Enterococcus faecium.
A partial vanG-like gene cluster encoding putative VanS(G), VanG, and VanT(G) proteins was also found.

Conclusions:

This study reports the first isolation of an anaerobic bacterium harboring the vanD gene cluster.
The novel strain also contains a vanG-like gene cluster, suggesting a dual resistance mechanism.
The presence of vanD and vanG genes in anaerobic gut bacteria highlights their potential as reservoirs for vancomycin resistance.
Further research is needed to understand the transmission dynamics and clinical implications of vancomycin resistance in anaerobic bacteria.