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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

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Related Experiment Video

Updated: Jul 12, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Flow cell within an LED: a proposal for an optical absorption detector.

Cássia Ugucione1, Arnaldo Alves Cardoso

  • 1Department of Analytical Chemistry, Institute of Chemistry, UNESP, CP 355, CEP 14801-970, Araraquara, SP, Brazil.

Analytical and Bioanalytical Chemistry
|August 29, 2007
PubMed
Summary

This study introduces a novel LED-based flow-through optical absorption detector for precise chemical analysis. The detector utilizes uniform liquid drops for controlled optical path length, enabling sensitive colorimetric determinations.

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Last Updated: Jul 12, 2026

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • Uniform liquid drops have long been utilized in instrumentation and chemical analysis.
  • Existing methods can face challenges with gas bubbles and particle precipitation.

Purpose of the Study:

  • To report the analytical application of a dynamic LED-based flow-through optical absorption detector.
  • To demonstrate the detector's feasibility using colorimetric determinations.

Main Methods:

  • A flow cell integrated with a high-intensity red LED (λmax = 630 nm) was developed.
  • Optical path length was controlled by continuous dropping of a solution.
  • The system was tested in a flow-injection setup for methylene blue and ammonium determination.

Main Results:

  • The detector successfully performed colorimetric determination of methylene blue and ammonium via Berthelot's reaction.
  • Ammonium determination yielded a detection limit better than 125 μg/L.
  • The flow cell demonstrated a favorable combination of optical path length, low volume, and fast washout.

Conclusions:

  • The developed LED-based detector offers a sensitive and efficient method for chemical analysis.
  • It provides a potential solution for optical detection challenges, including gas bubbles and turbidity.
  • The detector is suitable for advantageous use in automated analytical methods.