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Related Experiment Video

Updated: Jul 12, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Lsh controls Hox gene silencing during development.

Sichuan Xi¹, Heming Zhu, Hong Xu

¹Laboratory of Cancer Prevention, SAIC-Frederick, National Cancer Institute, Frederick, MD 21702-1201, USA.

Proceedings of the National Academy of Sciences of the United States of America

|August 30, 2007

Summary

Lsh, a DNA methylation regulator, controls Polycomb Repressive Complex (PRC) silencing of Hox genes during development. It forms a feedback loop reinforcing DNA methylation and gene silencing.

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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

Area of Science:

Epigenetics
Developmental Biology
Gene Regulation

Background:

Polycomb-mediated repression and DNA methylation are key epigenetic gene silencing mechanisms.
A link between Polycomb Repressive Complex (PRC) and DNA methyltransferases (Dnmts) is suggested in cancer.
The role of Lsh in normal embryonic gene silencing is not well understood.

Purpose of the Study:

To investigate the role of Lsh in Polycomb-mediated gene silencing during embryogenesis.
To determine if Lsh interacts with PRC components and influences DNA methylation.
To elucidate the physiological feedback loop involving Lsh, DNA methylation, and PRC targets.

Main Methods:

Chromatin immunoprecipitation assays to assess Lsh and PRC1 binding to Hox genes.
DNA methylation analysis of Hox gene loci.
Assessment of histone modifications associated with PRC-mediated silencing.

Main Results:

Lsh associates with specific Hox genes during development.
Lsh regulates the binding of Dnmt3b, DNA methylation, and silencing of Hox genes.
Lsh interacts with PRC1 components and influences PRC-mediated histone modifications.
Lsh participates in a feedback loop that strengthens DNA methylation and silencing of PRC targets.

Conclusions:

Lsh is a crucial regulator of PRC-mediated gene silencing in normal development.
Lsh integrates DNA methylation and Polycomb repression pathways.
Lsh plays a role in a feedback mechanism that ensures stable epigenetic silencing.