Related Experiment Video
Updated: Jul 12, 2026
Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
[Quantitative analysis of galangin from propolis in different areas by HPLC]
Xiao-Ping Wang1, Li Lin, Zhen-Lin Chen
1College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China.
Objective:
To establish an HPLC method for determination of galangin in propolis.
Methods:
HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm.
Results:
The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g).
Conclusion:
HPLC method is simple and reliable for determination of galangin in propolis.
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