Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Responsive polymer gels: double-stranded versus single-stranded DNA.

Diana Costa1, M Graça Miguel, Björn Lindman

  • 1Physical Chemistry 1, Centre for Chemistry and Chemical Engineering, Lund University, Box 124, S-22100 Lund, Sweden. diana.costa@fkem1.lu.se

The Journal of Physical Chemistry. B
|August 31, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From public healthcare centers to community pharmacies: the new Portuguese seasonal vaccination strategy.

Frontiers in public health·2026
Same author

Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges.

Methods (San Diego, Calif.)·2026
Same author

Modulation of interbrain synchrony by emotional valence and maternal presence in mother-child dyads: neural links to empathy and attachment.

Scientific reports·2026
Same author

The Potential of Chronotherapy and Nanotherapy-Based Strategies for Glioblastoma Treatment.

Pharmaceutics·2026
Same author

Development of RALA-Based Mannosylated Nanocarriers for Targeted Delivery of Minicircle DNA Vaccines Encoding HPV-16 Oncogenes.

Vaccines·2026
Same author

Influenza and COVID-19 vaccination intention in Portuguese adults from at-risk groups: a mixed-method study.

BMC public health·2025

Cross-linked DNA gels contract with added electrolytes, showing responsiveness. Single-stranded DNA gels collapse more than double-stranded DNA gels, especially with surfactants, due to charge density and flexibility differences.

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Biophysics

Background:

  • Cross-linked DNA gels exhibit osmotic swelling and deswelling in response to electrolytes and cosolutes.
  • This deswelling behavior is linked to DNA-cosolute interactions, forming the basis for responsive DNA formulations.
  • Both single-stranded DNA (ss-DNA) and double-stranded DNA (ds-DNA) gels have potential applications, and comparing their responses aids in understanding underlying mechanisms.

Purpose of the Study:

  • To investigate the deswelling behavior of cross-linked DNA gels in response to various cosolutes.
  • To compare the deswelling responses of ss-DNA and ds-DNA gels.
  • To elucidate the influence of DNA conformation, molecular weight, and cosolute properties on gel behavior.

Main Methods:

Related Experiment Videos

  • Preparation of cross-linked ss-DNA and ds-DNA gels.
  • Induction of denaturation in ds-DNA gels via heating and cooling, monitored by fluorescence with ethidium bromide.
  • Investigation of gel swelling/deswelling upon addition of diverse cosolutes (metal ions, polyamines, proteins, surfactants) across varying DNA molecular weights and conformations.

    • Main Results:

    • DNA molecular weight had minimal impact on deswelling, while conformation significantly affected it.
    • ss-DNA gels showed greater collapse than ds-DNA gels, particularly with cations and surfactants, attributed to differences in linear charge density, flexibility, and hydrophobicity.
    • Surfactant-induced deswelling varied; ss-DNA gels exhibited more pronounced "skin" formation (surface collapse) compared to ds-DNA gels, linked to deformation energy and chain stiffness.

    Conclusions:

    • The deswelling of DNA gels is a reversible process influenced by DNA conformation and cosolute interactions.
    • Differences in ss-DNA and ds-DNA gel responses stem from inherent structural and charge properties.
    • Understanding these interactions is crucial for designing tunable, responsive DNA-based materials.