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Related Experiment Videos

Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray.

Johan Vallon-Christersson1, Johan Staaf, Anders Kvist

  • 1Department of Oncology, Institute of Clinical Sciences, and SWEGENE DNA microarray resource center, Lund University, Barngatan 2:1, SE-221 85 Lund, Sweden. johan.vallon-christersson@med.lu.se

BMC Genomics
|August 31, 2007
PubMed
Summary
This summary is machine-generated.

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Antisense transcription, the expression of complementary DNA strands, is common in human cells, with up to 88% of genes showing this activity. However, this widespread antisense transcription has minimal impact on gene expression data from standard microarrays.

Area of Science:

  • Genomics
  • Molecular Biology
  • Gene Expression Analysis

Background:

  • Recent research indicates that many mammalian protein-coding genes also transcribe their complementary strands.
  • This phenomenon raises concerns about the accuracy of data from double-stranded complementary DNA (cDNA) microarrays due to potential dual-strand hybridization.

Purpose of the Study:

  • To experimentally determine the frequency of antisense transcription in human cells.
  • To assess the impact of antisense transcription on protein-coding expression patterns analyzed using double-stranded microarrays.

Main Methods:

  • Strand-specific cDNA microarrays were employed to profile sense and antisense transcription independently.
  • Experimental analysis of antisense transcription incidence and its influence on gene expression.

Related Experiment Videos

Main Results:

  • Concurrent expression from the complementary strand was observed in up to 88% of expressed protein-coding loci.
  • Antisense transcription is cell-specific and generally correlates positively with the expression of its sense counterpart.
  • Despite widespread occurrence, antisense signals exhibited a limited distorting effect on sense expression profiles obtained with double-stranded probes.

Conclusions:

  • Antisense transcription in humans is more prevalent than previously thought.
  • The influence of antisense transcription on expression profiles from conventional cDNA probes is limited.
  • This limited influence may be attributed to coordinated sense-antisense expression variations and a technical bias favoring sense-strand hybridization.