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Related Concept Videos

Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

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Related Experiment Video

Updated: Jul 12, 2026

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
06:04

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

Predicting human intestinal permeability using single-pass intestinal perfusion in rat.

Parvin Zakeri-Milani1, Hadi Valizadeh, Hosnieh Tajerzadeh

  • 1Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques
|August 31, 2007
PubMed
Summary

The single-pass intestinal perfusion (SPIP) technique in rats accurately predicts human intestinal drug absorption and permeability. This method reliably estimates fraction absorbed for oral drug formulations.

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Last Updated: Jul 12, 2026

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09:24

Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model

Published on: February 11, 2021

Area of Science:

  • Pharmacokinetics and drug absorption research.
  • In vivo preclinical models for drug development.

Background:

  • Accurate prediction of human intestinal permeability is crucial for oral drug development.
  • Preclinical models are essential for evaluating drug absorption characteristics before human trials.

Purpose of the Study:

  • To predict human intestinal permeability and fraction absorbed for oral doses.
  • To validate the single-pass intestinal perfusion (SPIP) technique in rats for human predictions.

Main Methods:

  • Determined permeability coefficients (Peff) for 14 compounds in anesthetized rats using SPIP.
  • Utilized phenol red as a non-absorbable marker to correct for water flux.
  • Quantified drug concentrations via HPLC and calculated Peff values.

Main Results:

  • Rat Peff values showed a 12.5-fold difference among compounds.
  • A strong correlation was observed between rat and human Peff (R²=0.93, P<0.0001).
  • Predicted human fraction absorbed (Fa) with high accuracy (R²=0.91, P<0.0001).

Conclusions:

  • SPIP in rats is a precise method for predicting human intestinal permeability.
  • SPIP reliably estimates the fraction of oral dose absorbed in humans for various formulations.