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Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
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Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle.

Bernard Couvreur1, Carine Letellier, Fabrice Olivier

  • 1Department of Virology, Veterinary and Agrochemical Research Centre, Bruxelles, Belgium. bcouvreu@ulb.ac.be

Veterinary Research
|August 31, 2007
PubMed
Summary

DNA vaccination using optimized glycoprotein E2 constructs against bovine viral diarrhoea virus (BVDV) in cattle successfully generated neutralizing antibodies. This approach shows promise for controlling BVDV infections in cattle herds.

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Area of Science:

  • Veterinary Virology
  • Molecular Immunology
  • Livestock Disease Control

Background:

  • Bovine viral diarrhoea virus (BVDV) causes significant economic losses in cattle globally.
  • Current BVDV control strategies face challenges due to viral genetic diversity.
  • Effective vaccines are crucial for managing BVDV infections.

Purpose of the Study:

  • To evaluate DNA immunization strategies for BVDV in cattle.
  • To assess the efficacy of plasmid constructs encoding glycoprotein E2 (E2) from BVDV-1 and BVDV-2.
  • To determine if the induced antibodies can neutralize circulating BVDV strains.

Main Methods:

  • Plasmid constructs encoding optimized E2.1 and E2.2 genes were designed.
  • A modified BoHV1 gD leader peptide was used for enhanced protein expression and export.
  • Recombinant E2 expression and epitope presentation were confirmed in COS7 cells.
  • DNA inoculation was performed in young cattle, followed by antibody analysis.

Main Results:

  • Optimized E2 coding sequences resulted in efficient protein expression in mammalian cells.
  • Recombinant E2 proteins presented native viral epitopes, confirmed by specific antisera.
  • DNA immunization in cattle elicited antibodies that neutralized major circulating BVDV genotypes.
  • The vaccine candidates demonstrated cross-neutralization capabilities.

Conclusions:

  • DNA immunization with optimized E2 constructs is a viable strategy for BVDV control.
  • The developed vaccine elicits broad-spectrum neutralizing antibodies against BVDV.
  • This approach offers a promising avenue for developing next-generation BVDV vaccines for cattle.