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Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

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High throughput workflow for coacervate formation and characterization in shampoo systems.

T H Kalantar¹, C J Tucker, A S Zalusky

¹Core R&D, The Dow Chemical Company,Midland, MI 48674, USA.

Journal of Cosmetic Science

|August 31, 2007

Summary

A new high-throughput workflow efficiently characterizes coacervate formation in shampoo formulations. This method saves time and materials, identifying optimal cationic cellulosic polymers and surfactants for improved hair benefits.

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Area of Science:

Cosmetic Science
Materials Science
Chemical Engineering

Background:

Cationic cellulosic polymers are key ingredients in shampoos, enhancing hair condition and active delivery.
Polymer deposition is linked to polymer/surfactant complex formation and phase separation (coacervation) upon dilution.
Traditional coacervate characterization is time-consuming and material-intensive.

Purpose of the Study:

To develop a semi-automated, high-throughput workflow for characterizing coacervate formation in shampoo systems.
To enable rapid screening of various cationic cellulosic polymers and surfactants for optimal coacervate behavior.
To reduce material consumption and testing time compared to standard methods.

Main Methods:

A simplified procedure for testing real-use shampoo dilutions was adapted to a liquid handling system.
Optimized liquid dispensing and mixing parameters for automated sample preparation.
Constructed haze diagrams for hundreds of formulations to quantify coacervate formation via haze measurements.

Main Results:

Successfully developed and validated a high-throughput workflow for coacervate characterization.
Identified optimal formulations and dilution levels exhibiting substantial coacervate formation.
Demonstrated that results from the high-throughput method correlate with standard haze and turbidity measurements.

Conclusions:

The developed high-throughput workflow offers significant time and material savings for characterizing coacervate formation.
This method facilitates the testing of a wider range of variables, accelerating the optimization of shampoo formulations.
Enables efficient identification of cationic cellulosic polymers and surfactants for enhanced hair care product performance.