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Updated: Jun 30, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Freezing and Viability of Tetrahymena pyriformis in Dimethylsulfoxide
Abstract:
Ciliated protozoa may be preserved at very low temperatures. Tetrahymena pyriformis, suspended in 10 percent dimethylsulfoxide, survived when frozen in two steps, first to -20 degrees C, then transferring immediately to a temperature of -196 degrees C. Motility and ability to reproduce were recovered after freezing by this method and subsequent storage for 3 months in a liquid nitrogen refrigerator.
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