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Related Experiment Video

Updated: Jul 12, 2026

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
08:31

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Potato protoplasts in crop improvement.

J F Shepard, D Bidney, E Shahin

    Science (New York, N.Y.)
    |April 4, 1980
    PubMed
    Summary

    Regenerating potato plants from single cells creates significant, stable variations in traits like tuber shape and disease resistance. This offers a promising avenue for potato varietal improvement.

    Area of Science:

    • Plant biotechnology
    • Agricultural science
    • Genetics

    Background:

    • Potato (Solanum tuberosum) is a vital global crop.
    • Asexual propagation is common in potato cultivation.
    • Genetic variation is crucial for crop improvement.

    Purpose of the Study:

    • To investigate the potential for generating novel potato variants.
    • To assess the stability of induced variations over generations.
    • To explore applications in potato breeding.

    Main Methods:

    • Regeneration of clonal populations from single-leaf cell protoplasts of 'Russet Burbank' potato.
    • Evaluation of horticultural traits (tuber shape, yield, maturity) over three tuber generations.
    • Assessment of disease resistance to early blight (Alternaria solani) and late blight (Phytophthora infestans).

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    Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
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    Published on: January 3, 2014

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    Last Updated: Jul 12, 2026

    Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
    08:31

    Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

    Published on: March 29, 2019

    High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato
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    Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
    07:33

    Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

    Published on: January 3, 2014

    Main Results:

    • High frequency of variation observed in horticultural and disease resistance traits.
    • Stable changes noted in tuber shape, yield, maturity, flowering photoperiod, and plant morphology.
    • Enhanced resistance to early and late blight consistently observed in regenerated populations.

    Conclusions:

    • Single-cell regeneration is a viable method for inducing stable genetic variation in potatoes.
    • This technique holds significant potential for potato varietal improvement.
    • Applications are particularly relevant for asexually propagated crops.