Related Experiment Video
Updated: Jul 9, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
Aggregation of HL-A Antigens at the Lymphocyte Surface Induced by Antiserum to beta2-Microglobulin
Abstract:
Rabbit antiserum to human beta(2)-microglobulin followed by goat antiserum to rabbit immunoglobulin induces aggregation of beta(2)-microglobulin at the lymphocyte surface, as shown by immunofluorescence and by acquisition of resistance to lysis by complement. This treatment also affects all HL-A antigens of the cell, which cap together with beta(2)-microglobulin, so that the cell becomes resistant to lysis upon addition of antibodies to HL-A in the presence of complement. These findings suggest that a physical linkage exists between these two classes of polypeptides at the surface of the living cell and is in agreement with recent biochemical data obtained by others with cell membrane preparations.
Related Concept Videos
Humoral Immune Responses
Blood Typing
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...
Cells of the Adaptive Immune Response
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Antibody Actions
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...

