Quantitation of estradiol receptors employing a sensitive radioimmunoassay

A method aimed at the identification and quantitation of estradiol receptors has been developed. The test is based on competitive binding reaction between solubilized cell receptors and anti-estradiol antibodies bound to acrylamide beads, for 3H-estradiol. The technique can be employed both for estradiol radioimmunoassay and quantitation of specific receptors in tissues and appears to be a sensitive, specific and reproducible method which can be applied to the evaluation of any hormone and its receptor.