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Related Experiment Videos

Isoenzyme changes in Candida albicans during domestication.

P F Lehmann1, L C Wu, D W Mackenzie

  • 1Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.

Journal of Clinical Microbiology
|November 1, 1991
PubMed
Summary

Candida albicans reference strains can change genetically over time in labs. Isoenzyme analysis revealed variations in glucose-6-phosphate dehydrogenase in some strains, indicating potential genetic instability during prolonged maintenance.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Candida albicans is a common fungal pathogen.
  • Reference cultures are crucial for consistent research.
  • Maintaining microbial cultures can sometimes lead to genetic drift.

Purpose of the Study:

  • To investigate potential genetic alterations in Candida albicans reference strains.
  • To compare isoenzyme profiles of different strains under prolonged laboratory maintenance.

Main Methods:

  • Isoenzyme analysis was conducted on multiple strains of Candida albicans.
  • Two reference cultures, B311 and NCPF3153, were analyzed.
  • Enzyme profiles, specifically glucose-6-phosphate dehydrogenase, were examined.

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Main Results:

  • Candida albicans B311 strains maintained consistent isoenzyme profiles.
  • Some strains derived from NCPF3153 were identical to B311 strains.
  • Other NCPF3153-derived strains exhibited variations in glucose-6-phosphate dehydrogenase isoenzymes.

Conclusions:

  • Prolonged laboratory maintenance can induce genetic alterations in Candida albicans.
  • Isoenzyme analysis is a valuable tool for detecting these genetic changes.
  • Variability in reference strains highlights the need for careful culture management.