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Related Experiment Videos

Progesterone binding by normal and abnormal human endometrium.

D T MacLaughlin, G S Richardson

    The Journal of Clinical Endocrinology and Metabolism
    |April 1, 1976
    PubMed
    Summary

    Researchers identified a progesterone-specific binding protein in human endometrial tissues. This protein

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    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Gynecology

    Background:

    • The human endometrium undergoes cyclical changes regulated by hormones, including progesterone.
    • Understanding progesterone's mechanism of action requires identifying its specific binding proteins.

    Purpose of the Study:

    • To investigate the presence and characteristics of a high-affinity, progesterone-specific binding protein in human endometrial cytosols.
    • To determine the presence of this binder in normal, abnormal, and cancerous endometrial tissues.

    Main Methods:

    • Cytosols from human endometrial tissue specimens were incubated with [3H]progesterone.
    • Binding affinity (Kd) and concentration were measured.
    • Protein nature and heat-lability were assessed using trypsin and heat treatment.

    Main Results:

    • A heat-labile, proteinaceous binder with high affinity for progesterone (Kd ~4.0 X 10(-10)M) was identified in normal and abnormal endometrial tissues.
    • Binder concentration varied cyclically, peaking at mid-cycle.
    • The binder was detected in some endometrial hyperplasias and carcinomas, but not in decidual tissues.

    Conclusions:

    • A progesterone-specific binding protein exists in the human endometrium, with cyclical variations.
    • The presence of this binder in hyperplasias and carcinomas suggests a potential role in endometrial pathology.

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