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Updated: Jul 12, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Deep-sea bacteria: isolation in the absence of decompression
Abstract:
Sampling and pure culture isolation of deep-sea bacteria without loss of in situ pressure is required in order to determine the viability of decompressionsensitive strains. This was achieved by using a pressure-retaining sterilizable seawater sampling system in connection with a prepressurized hyperbaric isolation chamber. Rates of growth and substrate uptake of the majority of isolates showed highly barotolerant characteristics, while the remainder (4 out of 15) exhibited barophilic characteristics.
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