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Related Experiment Videos

Microelectrode studies of toad urinary bladder epithelial cells using a novel mounting method.

P J Donaldson1, J P Leader

  • 1Department of Cellular and Molecular Biology, University of Auckland, New Zealand.

Pflugers Archiv : European Journal of Physiology
|November 1, 1991
PubMed
Summary

Researchers optimized microelectrode recordings in toad bladder epithelial cells by developing a stable basolateral impalement method. This technique improves the accuracy of membrane potential measurements in epithelial tissues.

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Area of Science:

  • Physiology
  • Membrane Biophysics
  • Epithelial Transport

Background:

  • Accurate measurement of epithelial membrane potentials is crucial for understanding cellular function.
  • Traditional microelectrode impalement methods face challenges with tissue stability and damage, particularly in tissues like toad bladders.
  • Optimizing impalement techniques is necessary to obtain reliable electrophysiological data.

Purpose of the Study:

  • To optimize microelectrode impalement conditions for recording stable membrane potentials in toad bladder epithelial cells.
  • To compare apical versus basolateral membrane impalement techniques.
  • To identify the most effective method for stable basolateral impalements.

Main Methods:

  • Short-circuited toad bladders were impaled via apical or basolateral membranes.

Related Experiment Videos

  • Two different methods for mounting bladders were tested to facilitate basolateral impalement.
  • Amiloride and Ba2+ were used to assess impalement damage with the agar method.

    • Main Results:

    • Apical membrane impalements resulted in biphasic potentials and were prone to damage.
    • Basolateral impalements yielded more stable and reliable membrane potential (Vsc) and resistance ratio (Ra/Rb) values (-57 mV and 5, respectively).
    • The agar mounting method provided the most stable basolateral impalements.

    Conclusions:

    • Basolateral microelectrode impalement of agar-mounted toad bladders is a viable and stable method.
    • This optimized technique makes toad bladder tissue more amenable to microelectrode studies.
    • The findings contribute to improved electrophysiological research in epithelial tissues.