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Related Experiment Video

Updated: Jun 26, 2026

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Published on: February 6, 2012

Chromatin structure: deduced from a minichromosome.

Science (New York, N.Y.)

|March 28, 1975

Summary

Simian virus 40-infected cells contain viral DNA as minichromosomes. These structures, segmented into 100-angstrom lengths with 200 base pairs each, resemble eukaryotic chromosomes and support current chromatin structure models.

Area of Science:

Molecular Biology
Virology
Cell Biology

Background:

Simian virus 40 (SV40) is a well-characterized DNA tumor virus used extensively in molecular biology research.
Understanding viral DNA organization within infected cells can provide insights into host cell processes and viral replication mechanisms.

Purpose of the Study:

To investigate the structural organization of simian virus 40 DNA within lytically infected cells.
To compare the structure of viral DNA to eukaryotic chromosomal organization.

Main Methods:

Analysis of DNA structures in simian virus 40-infected cells using electron microscopy or biophysical techniques (details not specified in abstract).
Characterization of DNA segment size and base pair composition.

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Last Updated: Jun 26, 2026

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

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Main Results:

Simian virus 40 DNA exists as discrete, small chromosomes (minichromosomes) in infected cells.
These minichromosomes are segmented into 100-angstrom lengths.
Each segment contains approximately 200 base pairs of DNA.

Conclusions:

The observed structure of simian virus 40 minichromosomes closely resembles that of eukaryotic chromosomes.
These findings are consistent with and support recently proposed models of chromatin structure.