Genotyping spring viraemia of carp virus and other piscine vesiculo-like viruses using reverse hybridisation
A M Sheppard1, R M Le Deuff, P D Martin
1Centre for Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, Weymouth, Dorset DT4 8UB, UK.
Abstract:
A simple nylon membrane-based DNA macroarray was developed to genotype spring viraemia of carp virus (SVCV) and related viruses. Twenty-six viruses were genotyped using the array, and the results were confirmed by phylogenetic analysis of a 426 bp partial glycoprotein gene sequence. The array was not only capable of discriminating between the 4 main genogroups of cyprinid vesiculo-type viruses described previously, but also accurately sub-type the SVC viruses assigned to Genogroup I. The assay offers a practical solution for diagnostic laboratories that currently lack a sequencing capability to confirm the nature of PCR products generated in suspected SVCV cases.
