Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Field Effect Transistor01:29

Field Effect Transistor

Field-effect transistors (FETs) are integral to electronic circuits and distinguished by their three-terminal setup: the gate, drain, and source. These transistors operate as unipolar devices, which utilize either electrons or holes as charge carriers, in contrast to bipolar transistors, which use both types of carriers. The primary function of the FET is to modulate the flow of these carriers from the source to the drain through a channel. The voltage difference between the gate and source...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of isoxazole-functionalized chitosan for enhanced anti-ulcer activity: From shrimp waste to therapeutic biopolymer.

International journal of biological macromolecules·2026
Same author

Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.

Micromachines·2026
Same author

An anti-fouling multifunctional interface enables hierarchical validation and ultrasensitive electrochemical detection of Coxsackievirus A6 specific nucleic acids.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Ultrasensitive electrochemical detection of glyphosate using a Pd-decorated Cit-HAP hybrid electrode.

Mikrochimica acta·2025
Same author

Morphological, Optical, and Dielectric Properties of Chitosan Biopolymer Thin Films Synthesized by Spray Pyrolysis.

ACS omega·2025
Same author

A Disposable Dopamine Sensor Based on Oxidized Cellulose Nanofibril-Modified SPCE.

Micromachines·2025

Related Experiment Video

Updated: Jul 12, 2026

Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing
10:45

Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing

Published on: August 29, 2025

Enzyme biosensors based on ion-selective field-effect transistors.

Sergei V Dzyadevych1, Alexey P Soldatkin, Anna V El'skaya

  • 1Laboratory of Biomolecular Electronics, Institute of Molecular Biology & Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, Kiev 03143, Ukraine. dzyad@yahoo.com

Analytica Chimica Acta
|September 1, 2007
PubMed
Summary

Ion-selective field-effect transistors (ISFETs) are crucial for bioanalytical applications. This work details ISFET principles, microfabrication, and enzyme biosensor development for future use.

More Related Videos

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Related Experiment Videos

Last Updated: Jul 12, 2026

Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing
10:45

Fabrication of a Solution-gated Indium-Tin-Oxide-based One-piece Transistor Enabling Sensitive Biosensing

Published on: August 29, 2025

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Microfabrication

Background:

  • Ion-selective field-effect transistors (ISFETs) are vital for bioanalytical measurements.
  • Understanding their theoretical principles and fabrication is key to advancing biosensor technology.

Purpose of the Study:

  • To discuss the theoretical underpinnings of ISFETs.
  • To explore microtechnologies for ISFET fabrication.
  • To detail measurement schemes and enzyme biosensor applications.

Main Methods:

  • Review of theoretical principles for ISFETs.
  • Discussion of microfabrication techniques.
  • Analysis of measurement setups and schemes.

Main Results:

  • Detailed exposition of ISFET theoretical principles.
  • Overview of modern microtechnologies for ISFET creation.
  • Description of enzyme biosensors based on ISFETs.

Conclusions:

  • ISFETs offer significant potential in bioanalytical practice.
  • Advancements in microfabrication enhance ISFET-based biosensor development.
  • Enzyme biosensors based on ISFETs show promising future applications.