Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 12, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Reconstruction of functional tissues with cell sheet engineering.

Joseph Yang1, Masayuki Yamato, Tatsuya Shimizu

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

Biomaterials
|September 1, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gelatin-based cryogels seeded with exosomes enhance osteogenic activity and bone regeneration in a rabbit femoral defect model.

Journal of biomaterials applications·2026
Same author

Reducing bias and enhancing equity in AI-enabled precision nutrition: addressing measurement error across wearables, multiomics, and dietary data.

Frontiers in digital health·2026
Same author

Real-World Health Care Resource Utilization and Economic Burden Among Patients with Idiopathic Pulmonary Fibrosis in Commercially Insured and Medicare Advantage Populations in the United States.

Journal of health economics and outcomes research·2026
Same author

Safety and Feasibility of IVC Flow Modulation With the Doraya in Acute Decompensated Heart Failure.

JACC. Heart failure·2026
Same author

Real-World Economic Burden Associated with Progressive Pulmonary Fibrosis in the US: An Administrative Claims Database Analysis.

PharmacoEconomics·2026
Same author

Integrative circulatory engineering for regenerative therapy: Dynamic perfusion design and metabolic maturation in cardiac tissue engineering.

Regenerative therapy·2026

Cell sheet engineering uses temperature-responsive polymers for controlled cell attachment and detachment. This novel approach creates functional tissue sheets and 3D tissues for regenerative medicine, offering an alternative to traditional methods.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Current tissue engineering relies on seeding cells into scaffolds, with mixed success in clinical trials.
  • Existing methods often combine biomaterials with living cells for tissue regeneration.

Purpose of the Study:

  • To develop a novel cell sheet engineering technique for creating functional tissues.
  • To enable controlled attachment and detachment of cells using temperature-responsive polymers.
  • To establish an alternative approach for regenerative medicine.

Main Methods:

  • Utilized culture surfaces grafted with poly(N-isoproplyacrylamide), a temperature-responsive polymer.
  • Developed methods for controlled cell attachment and detachment via temperature changes.

More Related Videos

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair
09:43

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

Related Experiment Videos

Last Updated: Jul 12, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair
09:43

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

  • Harvested cultured cell sheets for tissue fabrication.

    • Main Results:

    • Successfully created functional tissue sheets from temperature-responsive surfaces.
    • Demonstrated the application of cell sheet engineering for treating corneal dysfunction, esophageal cancer, tracheal resection, and cardiac failure.
    • Developed methods for generating 3D vascularized tissues and organ-like systems (heart, liver) using cell sheets and their extracellular matrix.

    Conclusions:

    • Cell sheet engineering offers a novel and effective strategy for regenerative medicine.
    • This approach allows for the creation of complex, functional tissue structures.
    • Provides a promising alternative to traditional tissue engineering methods.