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Related Experiment Video

Updated: Jul 12, 2026

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Published on: September 9, 2014

Differential gene expression during terminal erythroid differentiation.

S Koury¹, S Yarlagadda, K Moskalik-Liermo

¹Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, Buffalo, NY 14221, USA. stvkoury@buffalo.edu

|September 4, 2007

Summary

Researchers identified three novel genes, Apoll1, Xpo7, and D930015E06Rik, involved in terminal erythroid differentiation. One gene, D930015E06Rik, may also play a role in spermatogenesis.

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Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

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Published on: December 14, 2018

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Terminal erythroid differentiation is crucial for red blood cell formation but poorly understood.
Mechanisms governing erythroblast transformation remain largely unknown.

Purpose of the Study:

To identify novel genes involved in terminal erythroid differentiation.
To investigate the temporal expression patterns of these genes during differentiation.

Main Methods:

Differential display reverse transcriptase polymerase chain reaction (DD-RT-PCR) was employed.
Three differentially expressed cDNAs, designated LEB 1-3, were identified.

Main Results:

LEB-1 corresponds to Apoll1, LEB-2 to Xpo7 (a nuclear exportin), and LEB-3 to the novel gene D930015E06Rik.
Apoll1 shows homology to human apolipoprotein L family members.
D930015E06Rik is highly expressed in the testis, specifically in spermatocytes and spermatids.

Conclusions:

Three previously undescribed genes (Apoll1, Xpo7, D930015E06Rik) are implicated in terminal erythroid differentiation.
D930015E06Rik suggests a potential dual role in both erythropoiesis and spermatogenesis.
These findings provide new targets for understanding red blood cell development.