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A specific role for the C-terminal region of the Poly(A)-binding protein in mRNA decay

Ernesto Simón1, Bertrand Séraphin

  • 1Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, CNRS UPR2167 associée à l'Université P. et M. Curie, Avenue de la Terrasse, 91198 Gif sur Yvette, France.

Nucleic Acids Research
|September 4, 2007
PubMed

Insights

The poly(A)-binding protein Pab1

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • Polyadenylation is crucial for mRNA fate, influencing translation, export, and stability.
  • The poly(A)-binding protein Pab1 interacts with translation initiation factors and RNA.
  • Pab1 has distinct domains: RNA recognition motifs (RRMs) and a C-terminal region (linker and PABC domain).

Purpose of the Study:

  • To investigate the role of Pab1's C-terminal domains in mRNA metabolism.
  • To elucidate the mechanism by which Pab1 influences mRNA decay.

Main Methods:

  • Yeast genetics: creation and analysis of Pab1 C-terminal domain mutants.
  • In vivo assays: assessment of mRNA decay, export, and translation.
  • In vitro biochemical assays: deadenylation assays with purified complexes, binding assays.

Main Results:

  • Yeast mutants lacking Pab1's C-terminal domains exhibit synthetic interactions with mRNA decay mutants.
  • These Pab1 mutations inhibit mRNA decay by slowing deadenylation, without affecting mRNA export or translation.
  • The Pab1 linker domain is essential for regulating poly(A) tail deadenylation by the Pop2-Ccr4 complex.

Conclusions:

  • Pab1 directly participates in the regulation of mRNA decay.
  • The modular nature of Pab1 is highlighted, with different domains controlling distinct cellular processes.
  • Pab1's linker domain modulates poly(A) packaging, providing a mechanism to control mRNA decay rates.

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