Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental demonstration of optical cloaking of a free-standing Ag nanowire.

Optics letters·2026
Same author

Multiple Bone Metastases From Non-Muscle Invasive Bladder Cancer Responding to Combination Therapy With Enfortumab Vedotin and Pembrolizumab: A Case Report.

IJU case reports·2025
Same author

Eosinophils as a predictive marker of treatment-related adverse events in mRCC patients treated with first-line immune-checkpoint inhibitor combination therapy.

Scientific reports·2025
Same author

Clinical Outcomes of Neoadjuvant Paclitaxel/Cisplatin/Gemcitabine Compared with Gemcitabine/Cisplatin for Muscle-Invasive Bladder Cancer.

Acta medica Okayama·2025
Same author

A case of ureteral orifice obstruction by bladder indwelling catheter.

Urology case reports·2025
Same author

The Post-Polyketide Synthase Modification Mechanism in Hitachimycin Biosynthesis.

Chembiochem : a European journal of chemical biology·2024

Related Experiment Video

Updated: Jul 12, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Scanning electro-optic microscope based on surface plasmon resonance.

Shinsuke Sano1, Kazuma Tsuboi, Kotaro Kajikawa

  • 1Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8502, Japan.

Optics Letters
|September 4, 2007
PubMed
Summary

This study demonstrates an optical scanning probe microscope using surface plasmon resonance (SPR) to image polarization structures in hemicyanine monolayers. The technique effectively visualizes molecular organization on gold surfaces via electro-optic effects.

More Related Videos

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jul 12, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

Area of Science:

  • Materials Science
  • Optics
  • Surface Chemistry

Background:

  • Self-assembled monolayers (SAMs) are crucial in nanotechnology.
  • Understanding molecular polarization is key for advanced materials.
  • Optical microscopy techniques are vital for surface analysis.

Purpose of the Study:

  • To develop and demonstrate an optical scanning probe microscope for imaging polarization structures.
  • To investigate hemicyanine SAMs on gold surfaces.
  • To utilize surface plasmon resonance (SPR) for enhanced detection.

Main Methods:

  • Utilized an optical scanning probe microscope.
  • Employed surface plasmon resonance (SPR) in attenuated total reflection geometry.
  • Applied an AC field to induce the linear electro-optic effect in the monolayer.
  • Detected local refractive index changes via the AC component of reflected light intensity.

Main Results:

  • Successfully imaged polarization structures in hemicyanine SAMs on a gold surface.
  • SPR-based detection significantly intensified the AC component of reflected light.
  • Demonstrated clear imaging of polarization structures within the monolayer.

Conclusions:

  • The developed SPR-based optical scanning probe microscope is effective for imaging molecular polarization.
  • This technique provides high sensitivity for detecting local refractive index changes.
  • The method offers a clear pathway for visualizing complex polarization structures in monolayers.