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Related Experiment Videos

Trophic factor effects on septal cholinergic neurons.

C N Svendsen1, J D Cooper, M V Sofroniew

  • 1Department of Anatomy, Cambridge University, England.

Annals of the New York Academy of Sciences
|January 1, 1991
PubMed
Summary

Mature cholinergic neurons in the brain do not require target-derived nerve growth factor (NGF) or brain-derived neurotrophic factor (BDNF) for survival, but these factors are vital for maintaining their phenotype.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Neurotrophic Factors

Background:

  • The role of trophic factors in the adult central nervous system (CNS) is not well understood.
  • Cholinergic projections from the basal forebrain to the hippocampus may require nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) for normal adult CNS function.

Purpose of the Study:

  • To investigate the requirement of target-derived trophic factors for the survival and phenotype of cholinergic neurons in the adult CNS.
  • To determine if NGF and BDNF are necessary for maintaining the cholinergic phenotype and structural integrity of septal cholinergic neurons.

Main Methods:

  • Ablation of target neurons in the hippocampus that produce NGF and BDNF in young and aged rats.
  • In vitro culture of septal neurons to assess the effects of NGF withdrawal on cholinergic phenotype and cell survival.

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Main Results:

  • Ablation of NGF/BDNF-producing hippocampal neurons did not cause cholinergic neuron loss or phenotype loss in young rats.
  • Aged rats showed a reduction in cholinergic phenotype without cell death after target ablation.
  • In vitro NGF withdrawal led to down-regulation of enzymes, cell shrinkage, and reduced fiber density, but not death of aged cholinergic neurons.

Conclusions:

  • Target-derived factors like NGF and BDNF may not be essential for the survival of mature septal cholinergic neurons.
  • These trophic factors appear crucial for maintaining the cholinergic and structural characteristics of these neurons.