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Updated: Jul 12, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
[Aberrant methylation of APC and Bikunin CpG islands in sporadic breast carcinomas]
Yang-Lin Chen1, Yun-Tao Xie, Xian-Zi Wen
1Peking University School of Oncology and Beijing Institute for Cancer Research, Beijing 100036, China.
Objective:
To investigate relationship between methylation status of the APC and Bikunin CpG islands and clinicopathological characteristics of breast carcinomas.
Methods:
The methylation status of APC and Bikunin CpG islands in 152 sporadic breast carcinoma samples were analyzed by methylation specific PCR.
Results:
40.8% of breast carcinomas examined showed methylated signals for the APC. The methylation frequency of APC was significantly correlated to the tumor size (chi(2) = 4.041; P = 0.044), but not to patients' age, pathologic type of tumor, clinical stage, histological grade, lymph node metastasis and the status of estrogen or progestogen receptor. In addition, 24.6% of carcinoma samples examined revealed strong methylated signals for Bikunin. No significant correlation was found between the aberrant methylation of Bikunin and the clinicopathological characteristics of sporadic breast carcinomas.
Conclusion:
The aberrant methylations of APC and Bikunin are frequent events during breast carcinogenesis. APC methylation might play a role in the progression of breast cancer.
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