Related Experiment Video
Updated: Jul 12, 2026
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
Published on: March 12, 2022
[Fractal analysis of retinal vascular tree: segmentation and estimation methods]
Marcelo Bezerra de Melo de Mendonça1, Carlos Alexandre de Amorim Garcia, Romildo de Albuquerque Nogueira
1Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil. mendoncaoftalmo@yahoo.com.br
Purpose:
Although it has been proposed that retinal vasculature is fractal, no method of standardization has been performed for vascular segmentation or for dimension calculation, thus resulting in great variability among values of fractal dimensions. The present study was designed to determine if estimation of retinal vessel fractal dimensions is dependent on vascular segmentation and dimensional calculation methods.
Methods:
Ten eye fundus images were segmented to extract their vascular trees by four computational methods ("multi-threshold", "scale-space", "pixel classification" and "ridge based detection"). Their information, mass-radius and box counting fractal dimensions were calculated and compared with those of the same vascular trees manually segmented (gold standard).
Results:
The mean vascular tree dimension varied among the groups of different segmentation methods, from 1.39 to 1.47 for box counting, from 1.47 to 1.52 for information and from 1.48 to 1.57 for mass-radius dimensions. The utilization of different vascular segmentation methods and different dimension calculation methods introduced significant difference among fractal dimension of vessels.
Conclusion:
Estimation of retinal vessel fractal dimensions was dependent on both vascular segmentation and dimension calculation methods.
