Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluidity of human erythrocyte ghosts.

J Giannettini1, M Chauvet, M Dell'Amico

  • 1Laboratoire de Physique Pharmaceutique, UFR de Pharmacie, Marseille.

Biochemistry International
|July 1, 1991
PubMed
Summary

Human erythrocyte ghost fluidity, measured by order parameter (S) and rotation correlation time (tau c), is primarily influenced by cholesterol and protein content, not sex. Sickle cell disease impacts lipid bilayer motion.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19-associated Multi-Inflammatory Syndrome in Adults (MIS-A): look into the eyes!

Intensive care medicine·2022
Same author

Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor.

Scientific reports·2021
Same author

Solitonic waveguide reflection at an electric interface.

Optics express·2019
Same author

Voltage-induced waveguides in lithium niobate films on silicon substrates.

Optics letters·2017
Same author

Low drive voltage electro-optic Bragg deflector using a periodically poled lithium niobate planar waveguide.

Optics letters·2016
Same author

Telomeres and chromosomal instability in chronic lymphocytic leukemia.

Leukemia·2012

Area of Science:

  • Biophysics
  • Cell Biology
  • Membrane Biophysics

Background:

  • Erythrocyte membrane fluidity is crucial for cell function.
  • Understanding erythrocyte ghost composition and biophysical properties provides insights into membrane dynamics.

Purpose of the Study:

  • To investigate the factors affecting the fluidity of human erythrocyte ghosts.
  • To correlate fluidity parameters with biochemical composition and enzyme activity.
  • To examine the impact of sickle cell disease on erythrocyte membrane fluidity.

Main Methods:

  • Measurement of erythrocyte ghost fluidity using order parameter (S) and rotation correlation time (tau c).
  • Quantification of ghost protein, cholesterol, and phospholipid content.
  • Assay of acetylcholinesterase activity.
  • Preparation and analysis of liposomes from ghost lipid extracts.

Main Results:

  • No significant sex-based differences in erythrocyte ghost fluidity or composition were found.
  • Ghost order parameter (S) variations were mainly attributed to changes in cholesterol (61%) and protein content.
  • No correlation was observed between order parameter values and acetylcholinesterase activity.
  • Liposomes from ghost lipids exhibited lower order parameters than intact ghosts.
  • Sickle cell disease was associated with reduced bilayer lipid motionnal freedom, indicated by increased order parameter values.

Conclusions:

  • Cholesterol and protein content are key determinants of erythrocyte ghost order parameter.
  • Acetylcholinesterase activity is independent of membrane order parameter.
  • Sickle cell disease alters erythrocyte membrane dynamics, reducing lipid motionnal freedom.

Related Experiment Videos