Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Atomic force microscopy of supported planar membrane bilayers.

S Singh1, D J Keller

  • 1Department of Chemistry, University of New Mexico, Albuquerque 87131.

Biophysical Journal
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scanning force microscopy under aqueous solutions.

Current opinion in structural biology·1997
Same author

Topographical structure of membrane-bound Escherichia coli F1F0 ATP synthase in aqueous buffer.

FEBS letters·1996
Same author

Acute cardiotoxic effects of cocaine and a hyperadrenergic state in anesthetized dogs.

International journal of cardiology·1994
Same author

Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice.

Biochimica et biophysica acta·1992
Same author

"Pseudo-resistant" malaria in tropical countries.

Lancet (London, England)·1992
Same author

Effect of PEG 400, ethanol and laurocapram (Azone) on the transport of testosterone through rat skin.

Die Pharmazie·1992

Atomic Force Microscopy imaged lipid bilayers of DPPC and DPPE on mica. Different deposition methods yielded varied membrane structures, showing potential for imaging membrane proteins.

Area of Science:

  • Biophysics
  • Materials Science

Background:

  • Atomic Force Microscopy (AFM) is a powerful tool for imaging biological membranes at the nanoscale.
  • Understanding lipid bilayer formation on solid substrates is crucial for developing biosensing and biomimetic platforms.

Purpose of the Study:

  • To investigate the adsorption and imaging of dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidylethanolamine (DPPE) lipid bilayers on mica substrates using AFM.
  • To compare two different methods for preparing lipid membranes on mica for AFM analysis.
  • To assess the potential of these supported lipid bilayers as platforms for imaging membrane proteins.

Main Methods:

  • Preparation of lipid bilayers using two methods: dialysis of lipid-detergent solutions and adsorption of lipid vesicles onto freshly cleaved mica.

Related Experiment Videos

  • Imaging of the adsorbed lipid bilayers using Atomic Force Microscopy (AFM) in air.
  • Epifluorescence light microscopy was used to confirm lipid distribution.

    • Main Results:

    • AFM imaging revealed lipid bilayers adhering to the mica surface in either isolated patches or continuous sheets, dependent on deposition conditions.
    • Epifluorescence microscopy corroborated the AFM observations of lipid distribution.
    • Unfused vesicles bound to the substrate were observed with DPPE, demonstrating potential for protein immobilization.

    Conclusions:

    • Supported lipid bilayers of DPPC and DPPE can be successfully prepared and imaged on mica using AFM.
    • The deposition method significantly influences the morphology of the supported lipid bilayers.
    • These supported membranes show promise as natural anchors for AFM-based imaging of membrane proteins.