Related Experiment Video

Updated: Jul 12, 2026

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
09:12

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Selective Viral and Rickettsial Serum Antibody Absorption by a Chromatographic Column

N K Brown

    Science (New York, N.Y.)
    |February 3, 1961
    PubMed

    Serum antibodies behave as cations at neutral pH and thus have low affinity for cellulose anion-exchange columns. Antigens of small size derived from adenovirus, influenza virus, and typhus rickettsiae, however, readily adsorbed to such columns. These adsorbed antigens specifically removed antibodies from antisera. This simple method permits antibody absorption by antigens ordinarily sedimented with difficulty.

    More Related Videos

    An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay09:14

    An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay

    Published on: January 26, 2019

    In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses12:23

    In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

    Published on: September 7, 2022

    Related Concept Videos

    Affinity Chromatography01:03

    Affinity Chromatography

    Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...