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[Effects of growth factors on growth of stromal CFU-f in mouse bone marrow cell cultures]
Abstract:
Purified mouse IL-1 at doses 15-100 mu/ml inhibits the growth of stromal clonogenic cells /CFU-f/ both in full bone marrow cell cultures /F-cultures/ and in adherent bone marrow cell cultures /A-cultures/. Rec. human TNF-alpha inhibits growth of these cells at doses greater than 50 u/ml, but stimulates it /in 1.5 fold increase/at low doses /0.1-20 u/ml/ in cultures of both types. Rec. mouse IL-3 at doses 0.8-50 mu/ml slightly increases/in 1.6 fold increase/the in vitro growth of CFU-f and inhibits it at low doses in F-cultures. In A-cultures this factor stimulates CFU-f growth at all doses tested, but this stimulating effect takes place if only explantation density of mouse bone marrow cells in sufficiently high.
Insights
Interleukin-1 inhibits stromal cell growth, while TNF-alpha shows dual effects. Interleukin-3 also impacts stromal cell proliferation differently based on dose and culture conditions.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Stromal cells, including colony-forming unit-fibroblast (CFU-f), are crucial for hematopoiesis.
- Cytokines play a significant role in regulating bone marrow stromal cell function.
Purpose of the Study:
- To investigate the effects of purified mouse interleukin-1 (IL-1), recombinant human tumor necrosis factor-alpha (TNF-alpha), and recombinant mouse interleukin-3 (IL-3) on CFU-f growth in vitro.
- To determine the dose-dependent and culture-specific responses of CFU-f to these cytokines.
Main Methods:
- Bone marrow cell cultures were established using both full bone marrow (F-cultures) and adherent bone marrow cells (A-cultures).
- Purified mouse IL-1, recombinant human TNF-alpha, and recombinant mouse IL-3 were added to cultures at various concentrations.
- CFU-f growth was assessed in response to cytokine treatment.
Main Results:
- Mouse IL-1 (15-100 mu/ml) inhibited CFU-f growth in both F-cultures and A-cultures.
- Human TNF-alpha inhibited CFU-f at doses >50 u/ml but stimulated growth (1.5-fold) at lower doses (0.1-20 u/ml) in both culture types.
- Mouse IL-3 (0.8-50 mu/ml) slightly increased in vitro CFU-f growth (1.6-fold) in A-cultures and at higher doses in F-cultures, but inhibited it at low doses in F-cultures.
- The stimulatory effect of mouse IL-3 in A-cultures was dependent on sufficiently high explantation density.
Conclusions:
- IL-1 demonstrates inhibitory effects on CFU-f proliferation.
- TNF-alpha exhibits a dose-dependent biphasic effect on CFU-f, with inhibition at high doses and stimulation at low doses.
- IL-3 shows a complex regulatory role in CFU-f growth, with stimulatory effects in A-cultures dependent on cell density and inhibitory effects at low doses in F-cultures.