Related Experiment Video
Updated: Jul 12, 2026

07:07
A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
Published on: June 30, 2023
Incompatibility on brassica stigmas is overcome by treating pollen with cycloheximide
Abstract:
Pollen of Brassica exhibited strong self-incompatibility. It did not germinate on, adhere to, or extend into the stigmatic tissues of the female parent plant. In contrast, pollen that had been treated with cycloheximide germinated on and penetrated into self-stigmas to the same degree as untreated pollen placed on crossstigmas (compatible). Germ tubes from cycloheximide-treated pollen did not continue growth through stylar tissues and therefore were unable to effect fertilization.

