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Related Experiment Videos

TNF alpha mRNA expression in allergic inflammation.

S Ying1, D S Robinson, V Varney

  • 1Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, U.K.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
|November 1, 1991
PubMed
Summary

Tumor necrosis factor-alpha (TNF alpha) messenger RNA was found in allergic inflammation sites in atopic individuals. Increased TNF alpha mRNA in skin, nose, and bronchoalveolar lavage suggests its role in atopic allergic inflammation.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Allergy Research

Background:

  • Atopic allergic inflammation involves complex cellular and molecular responses.
  • Tumor necrosis factor-alpha (TNF alpha) is a key cytokine implicated in inflammatory processes.

Purpose of the Study:

  • To investigate the presence and localization of TNF alpha mRNA in allergic inflammation.
  • To compare TNF alpha mRNA levels in atopic subjects versus controls.

Main Methods:

  • In situ hybridization was used to detect TNF alpha mRNA.
  • Samples included skin and nasal biopsies from allergen-challenged atopic subjects.
  • Bronchoalveolar lavage (BAL) fluid from asthmatic and normal individuals was analyzed.

Main Results:

Related Experiment Videos

  • Significantly increased TNF alpha mRNA-positive cells were found in allergen-induced late phase reactions (LPR) of the skin and nose.
  • Higher frequencies of TNF alpha mRNA-positive cells were observed in BAL from atopic asthmatics compared to normal controls.
  • Positive signals for TNF alpha mRNA were detected in most biopsies and BAL samples.

Conclusions:

  • TNF alpha mRNA is present in cells infiltrating allergic inflammatory sites in atopic subjects.
  • Elevated TNF alpha mRNA levels in skin, nose, and airways suggest a significant role for TNF alpha in atopic allergic inflammation.