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Related Experiment Videos

Determination of apolipoprotein E phenotypes from stored or postmortem serum samples.

T Lehtimäki1

  • 1Department of Biomedical Sciences, University of Tampere, Finland.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 16, 1991
PubMed
Summary

Apolipoprotein E (Apo E) phenotyping from serum stored for 8 years showed significant inaccuracies, particularly for the Apo E4/4 phenotype. Fresh samples are recommended for reliable Apo E phenotyping.

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Area of Science:

  • Biochemistry
  • Genetics
  • Forensic Science

Background:

  • Apolipoprotein E (Apo E) plays a crucial role in lipid metabolism and is associated with various diseases.
  • Accurate Apo E phenotyping is essential for clinical diagnostics and research.
  • The stability of Apo E phenotypes in stored biological samples is not well-established.

Purpose of the Study:

  • To evaluate the reliability of Apo E phenotyping from long-term stored serum samples.
  • To assess the impact of the early postmortem interval on Apo E phenotype determination.

Main Methods:

  • Apolipoprotein E (Apo E) phenotyping was performed on fresh serum samples and serum stored for 8 years at -20°C from 42 individuals.
  • Apo E phenotyping was also conducted on serum samples collected from eight cadavers at 2, 6, 12, and 24 hours postmortem.

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  • Isoelectric focusing and immunoblotting techniques were utilized for Apo E phenotyping.

    • Main Results:

    • Apo E phenotyping from cadaver samples yielded consistent results across the 24-hour postmortem interval.
    • After 8 years of storage, 17% (7/42) of Apo E phenotypes were incorrectly assessed.
    • Specifically, Apo E4/4 phenotypes were often misidentified as Apo E4/3, and some Apo E4/3 and Apo E4/2 samples were falsely interpreted as Apo E3/3.

    Conclusions:

    • Long-term storage of serum samples (8 years at -20°C) significantly compromises the accuracy of Apolipoprotein E (Apo E) phenotyping.
    • The Apo E4/4 phenotype appears particularly susceptible to degradation or alteration during storage.
    • Fresh serum samples are recommended for accurate Apo E phenotyping to ensure reliable diagnostic and research outcomes.