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Related Experiment Videos

Antibody engineering: an overview.

R O'Kennedy1, P Roben

  • 1School of Biological Sciences, Dublin City University, Ireland.

Essays in Biochemistry
|January 1, 1991
PubMed
Summary
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Antibody engineering allows gene manipulation for creating humanized antibodies. This technology enables large-scale antibody production and novel applications in medicine and biosensing.

Area of Science:

  • Biotechnology
  • Immunology
  • Molecular Biology

Background:

  • Advances in genetic engineering now permit the isolation and manipulation of antibody genes.
  • The ability to modify antibodies has opened new avenues in biological research and therapeutic development.

Purpose of the Study:

  • To highlight the capabilities and applications of antibody engineering.
  • To discuss the production of various engineered antibody formats and their conjugates.

Main Methods:

  • Gene isolation and manipulation techniques for antibody production.
  • Humanization of mouse antibodies to create chimaeric or reshaped antibodies.
  • Development of novel expression systems for antibody production.

Main Results:

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  • Successful humanization of mouse antibodies, yielding chimaeric and reshaped antibodies.
  • Production of active antibody fragments, bifunctional, single-domain, and catalytic antibodies.
  • Generation of novel antibody conjugates, such as antibody-toxin and antibody-enzyme linked proteins.

Conclusions:

  • Antibody engineering is a versatile tool for large-scale antibody production.
  • Engineered antibodies offer significant potential in diverse applications including imaging, therapy, and biosensors.