Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Mass Spectrum: Interpretation

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

Mass Spectrometers

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:

Mass Spectrometry: Overview

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

Mass Analyzers: Overview

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Absolute Isotopic Abundance Ratios and Atomic Weight of a Reference Sample of Strontium.

Journal of research of the National Bureau of Standards (1977)·2021

Same author

JACK YOUDEN.

Journal of research of the National Bureau of Standards (1977)·2021

Same author

Absolute Isotopic Abundance Ratios and the Atomic Weight of a Reference Sample of Silicon.

Journal of research of the National Bureau of Standards. Section A, Physics and chemistry·2020

Same author

Atlantic salmon post-smolts adapted for a longer time to seawater develop an effective humoral and cellular immune response against Salmonid alphavirus.

Fish & shellfish immunology·2018

Same author

Time after seawater transfer influences immune cell abundance and responses to SAV3 infection in Atlantic salmon.

Journal of fish diseases·2018

Same author

Atlantic salmon adapted to seawater for 9 weeks develop a robust immune response to salmonid alphavirus upon bath challenge.

Fish & shellfish immunology·2018

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Laser resonance ionization mass spectrometry.

J D Fassett, L J Moore, J C Travis

Science (New York, N.Y.)

|October 18, 1985

Summary

Laser-based mass spectrometry enhances the detection of inorganic atomic species. This advanced technique improves sensitivity and selectivity for broader analytical chemistry applications.

Area of Science:

Analytical Chemistry
Atomic Spectroscopy
Mass Spectrometry

Background:

Traditional methods for inorganic atomic species analysis face limitations in sensitivity and selectivity.
Laser-based ionization techniques offer potential improvements over conventional atomic analysis.

Purpose of the Study:

To introduce and describe a laser-mass spectrometer system for inorganic atomic species analysis.
To highlight the advantages of laser ionization in mass spectrometry.

Main Methods:

Utilizing a tunable dye laser, with or without a pump laser, for resonant excitation of atomic species.
Employing mass spectrometry for the analysis of laser-induced ions.
Coupling laser systems with mass spectrometers to create an integrated analytical platform.

More Related Videos

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Published on: January 22, 2017

Related Experiment Videos

Last Updated: Jul 12, 2026

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Published on: January 22, 2017

Main Results:

Demonstration of laser-induced ionization of inorganic atomic species.
The laser-mass spectrometer system shows promise for enhanced sensitivity.
The system indicates improved selectivity in atomic species detection.

Conclusions:

The integration of lasers with mass spectrometers significantly advances inorganic atomic species analysis.
This laser-mass spectrometer system overcomes traditional analytical limitations.
Diverse applications in analytical chemistry are anticipated for this technology.