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Related Experiment Videos

Enterococcus faecalis: specific and non-specific interactions with human polymorphonuclear leukocytes.

C A Guzmàn1, C Pruzzo, L Calegari

  • 1Institute of Microbiology, University of Genoa, Italy.

FEMS Microbiology Letters
|November 15, 1991
PubMed
Summary
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Enterococcus faecalis interactions with human cells depend on infection source and growth conditions. Adhesins with specific sugars mediate these interactions, with serum growth impacting bacterial surface properties.

Area of Science:

  • Microbiology
  • Immunology
  • Cell Biology

Background:

  • Enterococcus faecalis exhibits varied adherence and internalization in human cells based on isolation source (UTI vs. endocarditis).
  • Bacterial growth in human serum modifies these interaction properties.

Purpose of the Study:

  • To investigate the role of specific adhesins in mediating interactions between E. faecalis and human polymorphonuclear leukocytes (PMNs).
  • To understand how growth in serum affects E. faecalis interactions with PMNs.

Main Methods:

  • Competition assays were employed to identify mediating adhesins.
  • E. faecalis strains from urinary tract infections (UTI) and endocarditis (EN) were cultured in brain-heart infusion broth (BHIB) and human serum.
  • Bacterial surface hydrophobicity was assessed.

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Main Results:

  • Adhesins with D-glucose mediated interactions between PMNs and serum-grown UTI isolates.
  • Adhesins with D-glucose and D-galactose were involved in interactions with serum-grown UTI isolates or EN isolates.
  • Serum growth reduced enterococcal surface hydrophobicity, impairing bacterial association with PMNs.

Conclusions:

  • Specific sugar moieties on E. faecalis adhesins dictate interactions with human PMNs.
  • Serum-induced changes in bacterial surface hydrophobicity influence E. faecalis-PMN interactions.