Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nano-Confined NHC-Al Interfaces for Efficient CO<sub>2</sub> Chemical Fixation.

ChemSusChem·2026
Same author

Evaluating surface interactions in supported imidazolium ionic liquid phases by using ultrasensitive DNP NMR and DFT studies.

Physical chemistry chemical physics : PCCP·2026
Same author

Unveiling the Mechanistic Role of Lewis Acidic Ionic Liquids in Styrene Polymerization.

ACS polymers Au·2026
Same author

Iron Age: Ionic-Liquid-Mediated Interfacial Charge Transfer Enables Selective CO<sub>2</sub> Photoreduction to Formic Acid on Iron Oxide.

Journal of the American Chemical Society·2026
Same author

Kinetics and Mechanism of the Enantioselective Passerini Multicomponent Reaction Catalyzed by Chiral Ionic Liquids.

ACS omega·2026
Same author

Ionic liquids: the chemistry beyond the ion pair.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026

Related Experiment Video

Updated: Jul 12, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

New sensitive fluorophores for selective DNA detection.

Brenno A D Neto1, Alexandre A M Lapis, Fabiana S Mancilha

  • 1Centro de Pesquisas em Biologia Molecular e Funcional (CPBMF), Tecnopuc, PUC-RS, Brazil. brenno.ipi@gmail.com

Organic Letters
|September 6, 2007
PubMed
Summary

New benzothiadiazole compounds act as highly sensitive "light up" probes for detecting duplex DNA. These probes offer improved spectrophotometric and spectrofluorimetric detection capabilities.

More Related Videos

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: Jul 12, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

  • Organic Chemistry
  • Molecular Biology
  • Biophysical Chemistry

Background:

  • Benzothiadiazole derivatives are explored for their photoluminescent properties.
  • Developing selective and sensitive probes for nucleic acid detection is crucial in molecular diagnostics.

Purpose of the Study:

  • To synthesize and characterize novel 4,7-disubstituted benzothiadiazoles.
  • To evaluate their potential as photoluminescent probes for duplex DNA detection.

Main Methods:

  • Synthesis of 4,7-disubstituted benzothiadiazoles with 1-arylethynyl and 4-methoxyphenyl groups.
  • Spectrophotometric and spectrofluorimetric measurements to assess probe performance.

Main Results:

  • The synthesized compounds exhibit selective "light up" fluorescence upon binding to duplex DNA.
  • Unprecedented sensitivity was achieved in both spectrophotometric and spectrofluorimetric assays.
  • The probes demonstrated high selectivity for duplex DNA over single-stranded DNA.

Conclusions:

  • 4,7-Disubstituted benzothiadiazoles are effective and highly sensitive probes for duplex DNA.
  • These probes offer a promising tool for sensitive detection in biochemical and diagnostic applications.