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Related Experiment Video

Updated: Jul 12, 2026

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
07:33

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Published on: May 26, 2021

Differences in stem and progenitor cell yield in different subcutaneous adipose tissue depots.

J K Fraser1, I Wulur, Z Alfonso

  • 1Cytori Therapeutics Inc, San Diego, CA 92121, USA. jfraser@cytoritx.com

Cytotherapy
|September 6, 2007
PubMed
Summary

Harvesting stem cells from human adipose tissue is influenced by collection site. Hip tissue yields significantly more stem and progenitor cells than abdominal tissue, impacting cell recovery for research.

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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
07:35

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Published on: March 7, 2015

Area of Science:

  • Stem cell biology
  • Tissue engineering
  • Regenerative medicine

Background:

  • Human adipose tissue contains multipotent cells, similar to mesenchymal stromal cells.
  • Previous studies focused on cell biology, neglecting tissue harvest considerations.

Purpose of the Study:

  • To evaluate the impact of harvest method and site on stem and progenitor cell recovery from human adipose tissue.
  • To compare syringe versus pump-assisted liposuction for adipose tissue harvest.
  • To analyze stem cell yield from abdominal versus hip subcutaneous adipose tissue.

Main Methods:

  • Adipose tissue was collected from 10 donors via syringe and pump-assisted liposuction (abdomen and hips).
  • Tissue was processed using collagenase digestion.
  • Stem and progenitor cell presence was determined using clonogenic assays (CFU-F, CFU-AP).

Main Results:

  • Syringe liposuction yielded more adipocytes and a trend towards higher cell recovery in the depleted fraction.
  • Significant donor-to-donor variation in stem cell recovery was observed.
  • Hip adipose tissue yielded 2.3-fold more CFU-F and 7-fold higher CFU-AP frequency than abdominal tissue.

Conclusions:

  • The harvest site significantly influences the quantity of stem and progenitor cells in subcutaneous adipose tissue.
  • Hip adipose tissue is a superior source for stem cell recovery compared to abdominal tissue.
  • These findings are crucial for standardizing adipose tissue harvest protocols in research and clinical applications.