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A spectrophotometric assay for lipase activity utilizing immobilized triacylglycerols
1Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Branisovská, Ceské Budĕjovice, Czechoslovakia.
Journal of Biochemical and Biophysical Methods
|October 1, 1991
Abstract:
New substrates for the determination of lipase activity have been developed. Triacylglycerols were immobilized by adsorption on an appropriate carrier or adsorbent yielding a lipase substrate in a powder form. The adsorbed triacylglycerols were easily hydrolyzed by lipases present in a reaction mixture. The released fatty acids were extracted with benzene and converted to the corresponding Cu (II) salts (copper soaps) which were measured spectrophotometrically.