Related Experiment Videos
Application of bromodeoxyuridine immunohistochemistry to the rat temporomandibular joint
1Second Department of Oral and Maxillofacial Surgery, Nagasaki University, School of Dentistry, Japan.
Okajimas Folia Anatomica Japonica
|October 1, 1991
Abstract:
To detect S-phase cells in the decalcified paraffin-embedded temporomandibular joint (TMJ) tissues of the rat, bromodeoxyuridine (BrdUrd) immunohistochemistry was performed. Percardiac perfusion of 10% EDTA-2Na Tris buffer accelerated the decalcification. The use of the monoclonal antibody to BrdUrd enabled the visualization of the distribution of S-phase cells. This method is practical in studying cell kinetics and the specific proliferation mode of the TMJ.