Related Experiment Video
Updated: Jul 12, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
[Studies on chemical constituents from leaves of Sapium sebiferum]
Hong-qing Wang1, Chun-yang Zhao, Ruo-yun Chen
1Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Objective:
To study the chemical constituents from leaves of Sapium sebiferum.
Method:
The compounds were isolated and purified by silic gel column chromatography and preparative HPLC. The structures were identified by various spectral evidence.
Result:
Nine compounds were obtained and they were shikimic acid (1), kaempferol (2), quercetin (3), isoquercein (4), hyperin (5), kaempferol-3-O-beta-D-glucopyranoside (6), kaempferol-3-O-beta-D-glueopyranoside (7), gallic acid (8), rutin (9).
Conclusion:
Compounds 1, 5, 6 and 7 are isolated from this genus for the first time and compound 9 is isolated from this plant for the first time.

