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Published on: February 27, 2014
[Effects of micrometer compound rhizoma coptidis on nuclear factor-kappaB and inflammatory factors in rabbit fed with
Bin Wang1, Si-ming Guan, Xi-dong Li
1Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. wangbin721025@163.com
Objective:
To investigate the effects of the micrometer compound rhizoma coptidis on inflammatory factors and its possible mechanism in rabbit fed with high-lipid food.
Method:
The levels of CRP, IL-1 and TNF-alpha were all determinated by ELISA method. The mRNA and activity of NF-kappaB were determinated by RT-PCR and EMSA, respectively.
Result:
The level of CRP, IL-1 and TNF-alpha were significantly increased by feeding for 16 weeks with high-lipid diet in rabbit. It was significantly increased that the mRNA and the binging activity with DNA of NF-kappaB in thorax aorta of rabbits fed by high-lipid diet, too. The micrometer compound rhizoma coptidis can reverse the effects of high-lipid diet on CRP, IL-1, TNF-alpha and NF-kappaB.
Conclusion:
The micrometer compound rhizoma coptidis can inhibit the expression of inflammatory factor possibly through inhibitting the expression and activity of NF-kappaB.
Insights
Rhizoma coptidis compound reverses high-fat diet-induced inflammation in rabbits. It inhibits inflammatory factors like CRP, IL-1, and TNF-alpha by suppressing NF-kappaB expression and activity.
Area of Science:
- Pharmacology and Toxicology
- Molecular Biology
- Immunology
Context:
- High-fat diets induce inflammation and cardiovascular issues.
- Nuclear factor-kappa B (NF-kappaB) is a key regulator of inflammatory responses.
- Rhizoma coptidis is a traditional Chinese medicine with potential anti-inflammatory properties.
Purpose:
- To evaluate the anti-inflammatory effects of a micrometer compound derived from Rhizoma coptidis.
- To investigate the underlying mechanism involving NF-kappaB in rabbits fed a high-fat diet.
Summary:
- High-fat diet significantly elevated C-reactive protein (CRP), Interleukin-1 (IL-1), and Tumor Necrosis Factor-alpha (TNF-alpha) levels.
- Increased mRNA expression and DNA-binding activity of NF-kappaB were observed in the aorta of rabbits on a high-fat diet.
- Rhizoma coptidis compound administration reversed these inflammatory changes and NF-kappaB activation.
Impact:
- This study suggests Rhizoma coptidis compound as a potential therapeutic agent for managing diet-induced inflammation.
- Understanding the NF-kappaB pathway inhibition provides mechanistic insight into its anti-inflammatory action.
- Highlights the potential of traditional medicine in addressing modern health challenges like metabolic inflammation.