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Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
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PCR real time assays for the early detection of BKV-DNA in immunocompromised patients

Katia Marinelli1, Patrizia Bagnarelli, Gianni Gaffi

  • 1Istitito di Microbiologia e Scienze Biomediche, Università Politecnica delle Marche, Ancona, Italy.

The New Microbiologica
|September 7, 2007
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...