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Combined FT-Raman and SEM studies of the effects of Er:YAG laser irradiation on dentin
Luís Eduardo Silva Soares1, Erick B P S Resende, Aldo Brugnera
1Biomedical Vibrational Spectroscopy Laboratory, Research and Development Institute (IP&D), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, Brazil.
Objective:
This study evaluated the molecular and morphological changes on dentin elements after Er:YAG laser irradiation.
Background Data:
Spectroscopy studies reporting the effects of Er:YAG laser irradiation as an alternative to acid etching are needed to better understand the laser's effects.
Methods:
The occlusal one-third of the crown of six human third molars was removed. The dentin surface was schematically divided into areas corresponding to four surface treatment groups: control (group C): 37% phosphoric acid etching; group I: Er:YAG laser 80 mJ; group II: Er:YAG laser 120 mJ; and group III: Er:YAG laser 180 mJ. The analysis was performed by scanning electron microscopy (SEM) and Fourier transform Raman spectroscopy (FT-Raman) before and after the treatments. Raman data were submitted to ANOVA and Bonferroni tests.
Results:
The SEM photomicrographs revealed open dentin tubules in the control group. The molars from groups I, II, and III showed partially open dentin tubules. SEM images showed that the laser-irradiated dentin surface was not favorable to the diffusion of monomers. A significant reduction of the spectra relative intensity was observed in group III specimens.
Conclusions:
Er:YAG laser irradiation with 180 mJ could produce chemical changes in proteins, phosphate, and carbonate in dentin.
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