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Updated: Jul 12, 2026
08:01
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Published on: April 21, 2022
Mesothelin, a possible target for immunotherapy, is expressed in primary AML cells
Daniel Steinbach1, Masanori Onda, Astrid Voigt
1University Children's Hospital Jena, Germany. Daniel@Steinba.ch
European Journal of Haematology
|September 7, 2007
Summary
Mesothelin protein is found in acute myeloid leukemia (AML) cells, but the targeted therapy SS1(dsFv)PE38 was ineffective. Further research is needed to explore alternative mesothelin-targeting treatments for AML.
Area of Science:
- Oncology
- Immunotherapy
- Hematology
Background:
- Mesothelin is a cancer-specific target due to low normal tissue expression.
- Mesothelin expression in hematological malignancies remains largely uncharacterized.
- SS1(dsFv)PE38 is an anti-mesothelin immunotoxin in clinical trials.
Purpose of the Study:
- To investigate mesothelin expression in acute myeloid leukemia (AML).
- To assess the efficacy of SS1(dsFv)PE38 immunotoxin against AML cells.
Main Methods:
- Western blot analysis
- Immunocytochemistry
- Real-time PCR
- MTT assays
Main Results:
- Mesothelin protein expression was confirmed in pediatric AML cells.
- AML cells showed no sensitivity to the SS1(dsFv)PE38 immunotoxin.
- Despite mesothelin presence, the immunotoxin did not induce cell death.
Conclusions:
- Primary AML cells express mesothelin.
- SS1(dsFv)PE38 immunotoxin is not effective against mesothelin-positive AML cells.
- Alternative mesothelin-targeting strategies warrant investigation for AML treatment.
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