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Does Japanese encephalitis virus share the same cellular receptor with other mosquito-borne flaviviruses on the C6/36

Junping Ren1, Tianbing Ding, Wei Zhang

  • 1Department of Microbiology, Fourth Military Medical University, 17 Changle West Road, Xi'an, 710032, People's Republic of China. junpingr@fmmu.edu.cn

Virology Journal
|September 7, 2007
PubMed

Insights

Researchers identified heat shock cognate protein 70 (HSC70) as a potential cellular receptor for Japanese encephalitis virus (JEV) in mosquito cells. This finding advances understanding of JEV entry mechanisms and mosquito-borne flavivirus interactions.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Japanese encephalitis virus (JEV) is a significant mosquito-borne flavivirus.
  • Early JEV infection mechanisms and cellular receptors remain largely unknown.
  • Flaviviruses like JEV, West Nile virus (WNV), and Dengue virus (DV) share similar structures and vector-virus interactions.

Purpose of the Study:

  • To identify cellular receptors involved in JEV entry into mosquito cells.
  • To investigate potential common receptors for mosquito-borne flaviviruses on C6/36 cells.
  • To elucidate the early events of JEV infection.

Main Methods:

  • Co-immunoprecipitation assay to identify proteins binding JEV on C6/36 cells.
  • Mass spectrometry to identify the binding protein.
  • Literature review on heat shock protein 70 (HSP70) family proteins as viral receptors.

Main Results:

  • A 74-KDa protein on C6/36 cells was found to bind JEV.
  • Mass spectrometry suggested this protein is heat shock cognate protein 70 (HSC70) from Aedes aegypti.
  • HSC70 is proposed as a potential penetration receptor mediating JEV entry.

Conclusions:

  • HSC70 is identified as a likely cellular receptor for JEV in C6/36 mosquito cells.
  • HSC70 may mediate JEV entry through fusion with the cell membrane, potentially involving cation channel interactions.
  • This discovery provides insights into JEV pathogenesis and potential therapeutic targets.

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