Related Experiment Video
Updated: Jul 12, 2026

Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
The effect of resveratrol on a cell model of human aging
Maurizio Stefani1, M Andrea Markus, Ruby C Y Lin
1Basic & Clinical Genomics Laboratory, School of Medical Sciences and Bosch Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.
Abstract:
The natural polyphenol resveratrol stimulates sirtuins and extends lifespan. Here resveratrol inhibited expression of replicative senescence marker INK4a in human dermal fibroblasts, and 47 of 19,000 genes from microarray experiments were differentially expressed. These included genes for growth, cell division, cell signaling, apoptosis, and transcription. Genes involved in Ras and ubiquitin pathways, Ras-GRF1, RAC3, and UBE2D3, were downregulated. The changes suggest resveratrol might alter sirtuin-regulated downstream pathways, rather than sirtuin activity. Serum deprivation and high confluency caused nuclear translocation of the SIRT1-regulated transcription factor FOXO3a. Our data indicate resveratrol's actions might cause FOXO recruitment to the nucleus.
Related Concept Videos
The Effect of Aging on Tissues
Replicative Cell Senescence
Replicative Cell Senescence
Aging
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
Mitochondria
Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism